activation
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علوم وتكنولوجيا
Ligandspecific activation trajectories dictate GPCR signalling in cells
Biosensor construction Gibson assembly All cloning, except for site-directed mutagenesis, was conducted using Gibson assembly (NEBuilder HiFi DNA Assembly Master…
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علوم وتكنولوجيا
ZAK activation at the collided ribosome
Generation of the knockout cell line (ZAK and RACK1 knockout) HEK293T RACK1-knockout cells were generated by CRISPR–Cas9 (refs. 51,52). The…
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علوم وتكنولوجيا
Florigen activation complex forms via multifaceted assembly in Arabidopsis
Plant materials and growth conditions The Arabidopsis thaliana Columbia (Col-0) ecotype was used as the main experimental organism. Seeds of…
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