GPCR
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علوم وتكنولوجيا
Ligandspecific activation trajectories dictate GPCR signalling in cells
Biosensor construction Gibson assembly All cloning, except for site-directed mutagenesis, was conducted using Gibson assembly (NEBuilder HiFi DNA Assembly Master…
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علوم وتكنولوجيا
Designing allosteric modulators to change GPCR G protein subtype selectivity
Cell lines HEK293T/17 (CRL-11268, RRID: CVCL_1926) cells were obtained from the American Type Culture Collection (ATCC). G-protein-deficient HEK293 cells (ΔGNAS, ΔGNAL,…
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