thinsp
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العلوم و التكنولوجيا
Crossregulation of (2Fe
Protein purification Assays of (2Fe–2S) cluster assembly Fe–S cluster assembly reactions were performed under anaerobic conditions (< 2 ppm O2) in…
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العلوم و التكنولوجيا
Sterile
Experimental setup The KATRIN experiment measures the electron energy spectrum of tritium β-decay near the kinetic endpoint E0 ≈ 18.6 keV. The 70-m-long…
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العلوم و التكنولوجيا
Glasses
SBP-utilization analysis Owing to inherent SBP scarcity, existing 3D display approaches have been forced into static compromises, each emphasizing specific…
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العلوم و التكنولوجيا
Long
Study population and ethics statement For time point 1, stool samples were collected from six adult volunteers living in the…
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العلوم و التكنولوجيا
Inhibitory PD
Mice CD45.2 (C57BL/6J) and B6.GFP (C57BL/6-Tg(UBC-GFP)30Scha/J) mice were purchased from the Jackson Laboratory (strains 000664 and 004354, respectively); CD45.1 (B6.SJL…
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العلوم و التكنولوجيا
Slipknot
Fabrication of the slipknot All experiments were conducted at room temperature (21 °C). The loading rate was set at 10 mm min–1 during…
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العلوم و التكنولوجيا
Structural basis of regulated N
Antibodies The following primary antibodies were used: mouse anti-CCDC134 (Santa Cruz Biotechnology, #sc-393390, RRID: AB_3662100, 1:500); mouse anti-GRP94 (R&D Systems,…
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العلوم و التكنولوجيا
Prime editing
General methods DNA amplification was conducted by PCR using Phusion U Green Multiplex PCR Master Mix (ThermoFisher Scientific) or Q5…
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العلوم و التكنولوجيا
Electro
Materials and instruments All reagents and solvents used for the synthesis of the compounds were purchased from Aldrich and Acros…
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العلوم و التكنولوجيا
Synthetic α
aSyn expression Escherichia coli strain BL21(DE3) was transformed with pT7-7 aSyn WT vector43 from Addgene, (plasmid 36046) and plated onto…
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